Nutritional Models of Experimentally-Induced Subacute Ruminal Acidosis (SARA) Differ in Their Impact on Rumen and Hindgut Bacterial Communities in Dairy Cows

نویسندگان

  • Jan C. Plaizier
  • Shucong Li
  • Hein M. Tun
  • Ehsan Khafipour
چکیده

Effects of subacute ruminal acidosis (SARA) challenges on the bacteria in rumen fluid, cecal digesta, and feces of dairy cows were determined using 16S rRNA gene pyrosequencing and real-time quantitative PCR. Six non-lactating Holstein cows with cannulas in the rumen and cecum were used in a 3 × 3 Latin square arrangement of treatments. During the first 3 wk of each experimental period, cows received a control diet containing 70% forages on a dry matter (DM) basis. In wk 4 of each period, cows received one of three diets: (1) the control diet; (2) a diet in which 34% of the dietary DM was replaced with pellets of ground wheat and barley (GBSC); or (3) a diet in which 37% of dietary DM was replaced with pellets of ground alfalfa (APSC). Rumen fluid, cecal digesta and feces were collected on d 5 of wk 4 of each period and the composition of the bacterial community was studied. Rumen fermentation responses were reported in a companion study. Both SARA-inducing challenges resulted in similar digesta pH depressions (as shown by the companion study), and reduced bacterial richness and diversity in rumen fluid, but GBSC had the larger effect. None of the challenges affected these measures in cecal digesta, and only GBSC reduced bacterial richness and diversity in feces. Only GBSC reduced the abundance of Bacteroidetes in rumen fluid. Abundances of limited number of bacterial genera identified by 16S rRNA gene sequencing in the rumen, cecum and feces were affected by the GBSC. The APSC did not affect any of these abundances. Both challenges increased the abundances of several starch, pectin, xylan, dextrin, lactate, succinate, and sugar fermenting bacterial species in the rumen, cecum, and feces as determined by qPCR. Only GBSC increased that of Megasphaera elsdenii in the rumen. Both challenges decreased the abundance of Streptococcus bovis, and increased that of Escherichia coli, in cecal digesta and feces, with GBSC having the larger effect. These results showed that the SARA challenges caused moderate and reversible changes of the composition of the bacteria in the foregut and hindgut, with the greater changes observed during GBSC.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Subacute ruminal acidosis: prevalence and risk factors in Greek dairy herds

Subacute ruminal acidosis (SARA) represents one of the most important metabolic subclinical disordersof high producing dairy cows, having serious impact in both animal health and herd profitability. The aim ofthis study was to confirm the presence of SARA in Greek dairy herds and record its prevalence and riskfactors. Ruminal fluid samples, via rumenocentesis, were obtained from a total of 153 ...

متن کامل

Impact of Subacute Ruminal Acidosis (SARA) on Ruminal Microbiome, Lipopoly saccharide and Bioamine and Rumen Epithelial Health of Dairy Cows

Maximizing milk yield without impairing host health is a great challenge for dairy industry. However, the high concentrate feeding often unavoidably increases the risk of Subacute ruminal acidosis (SARA) in dairy cows, characterized by periodic episodes of low ruminal pH. The economic costs caused by SARA are enormous, while its pathogenesis is not very clear. As the first and the main digestiv...

متن کامل

Prevalence of subacute ruminal acidosis in some dairy herds of Khorasan Razavi province, northeast of Iran

To estimate the prevalence of subacute ruminal acidosis (SARA) in dairy cows, a total of 196 ruminalfluid samples were drawn by rumenocentesis from 10 dairy herds of Khorasan Razavi province, northeast ofIran. Two groups of 12 cows, early lactation and mid-lactation cows were sampled in each dairy herd andruminal pH was determined immediately using a portable pH-meter. A total of 54 cows (27.6%...

متن کامل

Minimizing the Risk for Rumen Acidosis

Ruminal acidosis is a common disease in high yielding dairy cows, during which the rumen pH is depressed for several hours per day. There is no general agreement of the definition of the disease, and its diagnosis based on rumen pH measurement is invasive and inaccurate. As a result, the disease often goes unnoticed, and its impact is not well recognized. Surveys suggest a prevalence of between...

متن کامل

Subacute Ruminal Acidosis and Evaluation of Blood Gas Analysis in Dairy Cow

Subacute Ruminal Acidosis (SARA) corresponds to an imbalance between lactate-producing bacteria and lactate-using bacteria, which results in a change in ruminal pH associated with a prevalent consumption of rapidly fermentable carbohydrates. In our study, 216 primiparus and multiparus dairy cows were selected from 20 Italian intensive dairy herds and were divided into three groups based on the ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره 7  شماره 

صفحات  -

تاریخ انتشار 2016